Year 2021
September 2021


Abstract Title
Evaluation of glucose interference in enzymatic creatinine measurement in two different peritoneal dialysis fluids.



MOH Holdings Pte Ltd (MOHH)1, Khoo Teck Puat Hospital2 

Background & Hypothesis

The accurate assessment of creatinine in the peritoneal equilibration test (PET) is essential for the planning of peritoneal dialysis (PD). We undertook this study to assess whether our hospital enzymatic creatinine assay (Roche Diagnostics) would be affected by the glucose content of two different PD dialysate fluids used for PET testing.


Roche calibrator material (baseline creatinine 336umol/L, glucose 11.1mmol/L) was combined with fixed volumes of deionised water/Dianeal/Balance PD fluid to create a series of samples to assess creatinine values with varying levels of glucose. Each sample run twice each day over 2 days for each level, and mean creatinine/glucose was derived for each level of dilution. This was then run on the serum mode of the Roche enzymatic Creatinine assay.


Seven levels of each concentration of Dianeal and Balance PD fluids were created. We compared the creatinine values at each level to the control values by ANOVA analysis. For Dianeal, the groups only had a significant difference from the control at the level of expected creatinine of 201.6umol/L (p=0.01) (glucose 6.4-86mmol/L). However, at subsequent higher glucose concentrations up to 153mmol/L, there was no further significant differences between the Dianeal-diluted and control samples (p=0.06-0.86). Balance-diluted samples had no significant differences with the controls at every level of dilution (p=0.11-0.93).

Discussion & Conclusion

No significant difference was found in creatinine measurements with increasing levels of glucose when compared to controls. We have demonstrated the lack of interference in two different kinds of PD fluids, up to glucose concentrations of 155mmol/L.